白桦花发育基因BpMADS3对玉米的遗传转化及功能验证
MADS-box基因编码多种具有重要生物学功能的转录调节因子，能够激活或抑制其他基因的转录，在调节植物根、叶、花和果实的发育，尤其在开花植物花的发育过程中起到重要的调控作用。本研究将东北白桦树中克隆得到的BpMADS3基因导入玉米中，验证其在玉米中的功能。结果表明：利用In-Fushion技术成功构建了BpMADS3基因的单子叶植物表达载体pTF101.1-T-nos-Ubi-BpMADS3；采用花粉管通道法将该基因导入玉米自交系合344中，获得5个T2代PCR和RT-PCR阳性株系；转基因株系H1-25和H1-76的散粉期和抽丝期均比对照提前，穗重、穗长、穗粗、和百粒重等产量相关性状显著或极显著高于对照。本研究结果初步证明BpMADS3基因的导入对玉米花期提前和产量增加有一定的功效。
英文摘要:
MADS-box genes，as a transcription factor, played a regulatory role in development of roots, leaves, flowers and fruit of plants by activating or inhibiting transcription of other genes. This study intends to identify the function of BpMADS3 gene cloned from Northeast Birch in maize. The results showed that BpMADS3 gene was successfully inserted into the over-expression vector by the In-fusion cloning technology, named pTF101.1-T-nos-Ubi-BpMADS3. The gene was transformed into maize inbred line He344 by pollen tube pathway. Five T2-generation lines were positive in PCR and TR-PCR test. The pollinating and silking date of transgenic line H1-25 and H1-76 were shortened compared to the control line. The ear weight, ear length, ear diameter, 100-grain weight of these two lines was significantly higher or great significantly higher than the control. The initial experiment proved that the introduction of BpMADS3 gene to maize could make the flowing earlier and increase the yield.
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