课题组前期已确定了玉米丝黑穗病苗期检测适宜的病原菌侵染条件、PCR检测技术、最佳检测时期及检测部位。在此基础之上,本研究以40份已知抗性级别的玉米自交系为试材,采用优化的苗期室内接种技术接种丝黑穗病菌并检测,将侵染率与历年田间平均发病率进行回归分析,进而确定该评价方法的抗性分级标准,最终组装形成完整的评价技术体系。结果表明：苗期室内接种病菌后40份玉米自交系的侵染率与历年平均田间接种发病率之间存在极显著的正相关,相关系数r=0.8570,回归方程为y = 0.0004x3 - 0.0667x2 + 3.8137x + 17.494；抗性分级标准包括,侵染率0%-20.0%为高抗,20.1%-40.0%为抗病,40.1%-50.0%为中抗,50.1%-90.0%为感病,90.1%-100%为高感。本研究结果为快速准确对玉米种质资源进行早期丝黑穗病抗性评价和筛选提供技术支持。
英文摘要:
In previous research, we have cleared three in four elements of PCR rapid identification technology of maize head smut resistance at seedling stage, including the suitable infection condition, PCRSdetection technology, and the best detection time and detection parts. In this study, 40 inbred lines with known resistance level were infected maize head smut by optimizing infection method indoor at seedling stage. Regression analysis was employed to treat the average incidence rate in the field inoculation and indoor inoculation. Then the resistance levelSevaluation standard of maize head smut indoor inoculation should be established, and be assembled to anSintegratedSevaluation system. The result showed that aSpositiveScorrelation was obtained between the average incidence rate of 40 inbred lines in the field inoculation and indoor inoculation. The correlationScoefficient was 0.8570, and regression equation was y = 0.0004x3 - 0.0667x2 + 3.8137x + 17.494. The resistance level evaluation standard was emerged based on different incidence rate, high resistance (0%-20.0%), resistance (20.1%-40.0%), moderate resistance (40.1%-50.0%), sensitivity (50.1%-90.0%), and high sensitivity (90.1%-100%). These results should provide the technicalSsupport to rapid evaluate and select maize germplasm with head smut resistance at early stage.
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