解淀粉芽孢杆菌B9601-Y2对玉米叶斑病的防治效果
解淀粉芽孢杆菌B9601-Y2是一株广泛应用的生防菌株。为了明确其对玉米叶斑病的防治效果，采用凹玻片法测定了Y2发酵液、上清液和芽孢对4种病害的孢子萌发抑制率，大斑病菌抑制率依次为82.72%、76.71%、4.89%，小斑病菌抑制率分别为88.04%、82.85%、5.63%，圆斑病菌抑制率分别为84.90%、79.90%、10.81%，弯孢霉叶斑病菌抑制率分别为98.79%、95.05%和6.23%。温室盆栽试验明确了Y2的最佳施用浓度为1×107CFU/mL，最佳施药时间为接种病菌前8h，治疗效果较差；对4种叶斑病的预防效果分别达80.83%、84.62%、84.33%和87.74%。田间试验表明Y2悬浮剂对4种病害的预防效果分别为65.90%、69.21%、67.97%和70.22%。
英文摘要:
Bacillus amyloliquefaciensY2 is a widely used biocontrol agent. In order to clarify its controlling effect on corn leaf spots, using concave slide method, conidia germination of the fungi(Exserohilum turcicum, Cochliobolus heterostrophus, Cochliobolus carbonum and Curvularia lunata) was determined with fermentation liquid, supernatant and Bacillus spores. The germination inhibition rates were 82.72%, 76.71% and 4.89% for E. turcicum; 88.04%, 82.85% and 5.63% for C. heterostrophus; 84.9%, 79.9% and 10.81% for C. carbonum; 98.79%, 95.05% and 6.23% for C. lunata, respectively. Through the greenhouse pot experiment, the suitable concentration of Y2 was 1 × 107CFU/ml, and the best application time was 8 hours before the pathogen inoculation. Preventive effect for the four leaf spot diseases with Y2 fermentation liquid were 80.83%, 84.62%, 84.33% and 87.74%, respectively, in the greenhouse. Correspondingly, the biocontrol agent Y2 SC could prevent 65.90%, 69.21%, 67.97% and 70.22% of the four diseases in the field though its curing effect was not as well as preventive one .
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