为研究玉米茎秆穿刺强度在不同种植密度下的遗传机制,以四287与四144为亲本组配的188个F2:3家系为材料,分别于2013年和2014年在2个种植密度下调查茎秆穿刺强度,并采用复合区间作图法进行QTL分析。结果表明,在2个种植密度下共检测到13个与茎秆穿刺强度相关的QTL,分布于玉米的第1、2、3、4、6、7、8和9染色体上,单个QTL可解释3.8%-11.8%的表型变异。其中,位于第6染色体检测到的QTL在所有环境中均能稳定表达。本研究结果将为玉米抗倒伏相关主效QTL的精细定位及分子标记辅助选择提供有利条件。
英文摘要:
To investigate the genetic basis for rind penetrometer resistance (RPR) under different plant densities in maize, an F2:3 population with 188 families from the cross between Si287 and Si144 was developed. The 188 F3 lines were evaluated for PRP under two plant densities in 2013 and 2014. QTL analysis was carried out to determine relationship between marker genotype and RPR evaluated using composite interval mapping (CIM). A total of 13 QTLs were identified on chromosomes 1, 2, 3, 4, 6, 7, 8 and 9, each QTL explained phenotypic variation ranging from 3.8% to 11.8%. Among these, the QTL on chromosome 6 for RPR was detected in each environment. The results in this study will facilitate fine mapping of major QTL underlying RPR as well as marker assisted selection in an applied breeding program.
;
