本研究依据GenBank数据库中发表的拟南芥DREB基因序列进行优化,人工合成了DREB基因序列,构建植物表达载体,通过农杆菌介导法将DREB基因转入玉米自交系HiII中,获得转DREB基因玉米材料,经过分子检测获得4个阳性转化事件。在不同浓度水平的NaCl 溶液(0、40、80、120 mmol/L)和Na2CO3 溶液(0、10、20、30 mmol/L)胁迫下,研究其耐盐碱性。结果表明：80 mmol/L 的NaCl溶液和20 mmol/L Na2CO3溶液可以作为转DREB基因玉米的耐盐碱分析条件；DREB基因已经整合到玉米基因组中,转DREB基因玉米的耐盐碱性得到了显著提高。获得的转DREB基因玉米丰富了耐盐碱玉米种质资源。
英文摘要:
This study based on the published in GenBank database of arabidopsis thaliana DREB gene sequence optimization, synthetic DREB gene sequences, the construction of plant expression vector, the DREB gene was transferred into maize inbred line HiII by Agrobacterium tumefaciens mediated transformation. Get 4 positive transformation events. Saline-alkali tolerance of transgenic maize carrying DREB was analyzed, under different saline-stress conditions(NaCl 0、40、80、120 mmol/L)and different alkali-stress conditions (Na2CO3 0、10、20、30 mmol/L). The main results were summarized that 80 mmol/L NaCl and 20 mmol/L Na2CO3 were the appropriate concentration in saline-alkali tolerance analysis on transgenic maize. The DREB was integrated into maize genome and the saline-alkali tolerance was improved. Transgenic maize could enrich saline-alkali tolerance germplasm of maize.
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