玉米磷素相关根系性状Meta-QTL及候选基因发掘
以玉米高密度遗传连锁图谱IBM2 Neighbors 20008为参考图谱，收集来自不同实验中的175个玉米磷相关根系数量性状位点（quantitative trait locus, QTL）信息，利用 Biomercator 2.1软件，构建出玉米磷相关根系QTL整合图谱；采用元分析技术，在10条染色体上发掘出26个与磷相关根系性状“一致性”QTL区间。图距范围在0.8-14.3cM之间。除2、5和8号染色体上仅检测到一个MQTL外，其余染色体上均检测到2-4个MQTL。根据“一致性”QTL区间两端标记在玉米物理图谱Ref Gen＿v2上的位置，将“一致性”QTL区间进行物理图谱定位。单个“一致性”QTL所在物理图谱上的图距范围在0.1-9.1Mb之间。在MQTL1、2、4、8、11、12、15、18、20、22中检测到15个与磷素吸收利用以及根系性状相关的候选基因，为深入理解磷素控制根系生长的遗传控制机制和分子设计育种提供科学依据。
英文摘要:
In order to unlock the full potential of the information contained in these independent experiments, 175 phosphrous related QTL in maize were collected from maize genomic database(maize GDB) and were compiled to construct the integration QTL map for phosphrous related root traits in maize (Zea mays L). The high-scale genetic linkage map of maize IBM2 Neighbors 2008 was used as reference and the map were constructed with BioMercator 2.1 software based on the method of meta analysis. Twenty six consistent MQTL for maize were found. The range of MQTL interval distance were 0.8-14.3cM, 2-4 MQTL were found in each chromosomes except in 2、5 and 8 chromosomes. According the both end of marker, the physical position of MQTL in Ref Gen_v2 of chromosomes was found. The range of each MQTL were 0.1-9.1Mb, a total of 15 candidate genes in the consensus QTL region were discovered and downloaded from plant GDB(http://www.plantgdb.org) in 10 MQTLs. The results suggested that this approach provided a useful tool for identifying QTL conferring phosphorus and other candidate gene in maize.
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