解淀粉芽孢杆菌B9601-Y2对玉米挥发物质的影响
解淀粉芽孢杆菌B9601-Y2是一株广泛应用的生防菌株。为了明确其防病机制,研究施用后对玉米挥发物质的影响,在盆栽条件下,研究了喷施Y2后,玉米植株释放挥发物的变化。利用顶空抽提法收集菌液和各处理玉米幼苗挥发物,并通过气相色谱-质谱联用仪对其进行分离和鉴定,共收集到18种Y2菌液挥发物组分和42种玉米活体植株挥发物组分。玉米释放醛类化合物6种,萜烯类15种,其中庚醛、十二烷、癸醛和十三烷为菌液和玉米植株共同抽提到的挥发物。不同处理收集的玉米活体植株挥发物组分种类和相对含量存在差异。Y2处理玉米1d、3d和5d后,玉米新出现了1-乙基-2-甲基环戊烷、2-辛烯醛、十一烷、十四碳烯和β-愈创木烯5种化合物,α-衣兰烯等11种化合物相对含量极显著地提高,芳樟醇等8种合化物出现振荡,反-罗勒烯等6种化合物消失；7d后各处理的玉米活体植株挥发物种类与相对含量均趋于对照。
英文摘要:
Bacillus amyloliquefaciens Y2 is a widely used biocontrol agent. To understand the possible mechanism for Y2 to control plant diseases, the dynamics of volatiles from maize plants were studied in pot experiment. Eighteen and forty-two components were collected from headspace of Y2 fermentation and maize plants by dynamic head-space absorption method and identified by gas chromatography coupled by mass spectrometry(GC-MS). The major components from maize were 6 aldehydes and 15 terpenes, among which four components, n-heptaldehyde, dodecane, n-decanal and n-tridecane were collected from both of Y2 liquid and maize. Volatiles of maize differed in species and relative content based on the different treatments．Maize with Y2 spray after 1, 3 and 5 days produced 5 new compounds including 1-ethyl-2-methyl cyclopentane,2-octenal, n-hendecane, 1-tetradecene and β-guaiene, and increased significantly 11 compounds including α-ylangene, nonaldehyde, (+)-cyclosativene, and so on,fluctuated 8 compounds including linalool and evanished 6 chemicals, for example, 3, 7-dimethyl-1, 3, 7-octatriene(E)-ocimene. All of volatiles of maize treated with Y2 recovered to and tended normal after 7 days.
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